Optimization of SPME–GC–MS and characterization of floral scents from Aquilegia japonica and A. amurensis flowers

Abstract Background The floral scents of plants play a key role in plant reproduction through the communication between and pollinators. Aquilegia as model species for studying evolution, however, there have been few studies on relationships pollination taxa. Methods In this study, three types solid-phase micro-extraction (SPME) fiber coatings (DVB/PDMS, CAR/PDMS, DVB/CAR/PDMS) were evaluated their performance extracting volatile organic compounds (VOCs) from flowers amurensis , which can contribute to future elucidating process. Results total, 55 VOCs identified, among them, 50, 47 45 extracted by DVB/CAR/PDMS fiber, CAR/PDMS DVB/PDMS fibers, respectively. Only 30 detected A. japonica Furthermore, relative contents 8 significant different (VIP > 1 p < 0.05) . Conclusions results be applied new chemical composition processes attraction pollinator. It may provide ideas rapid evolution frequent interspecific hybridization